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991.
Hong Wang Liping Hu Knut Dalen Heidi Dorward Amy Marcinkiewicz Deanna Russell Dawei Gong Constantine Londos Tomohiro Yamaguchi Cecilia Holm Mark A. Rizzo Dawn Brasaemle Carole Sztalryd 《The Journal of biological chemistry》2009,284(46):32116-32125
Lipolysis is an important metabolic pathway controlling energy homeostasis through degradation of triglycerides stored in lipid droplets and release of fatty acids. Lipid droplets of mammalian cells are coated with one or more members of the PAT protein family, which serve important functions in regulating lipolysis. In this study, we investigate the mechanisms by which PAT family members, perilipin A, adipose differentiation-related protein (ADFP), and LSDP5, control lipolysis catalyzed by hormone-sensitive lipase (HSL), a major lipase in adipocytes and several non-adipose cells. We applied fluorescence microscopic tools to analyze proteins in situ in cultured Chinese hamster ovary cells using fluorescence recovery after photobleaching and anisotropy Forster resonance energy transfer. Fluorescence recovery after photobleaching data show that ADFP and LSDP5 exchange between lipid droplet and cytoplasmic pools, whereas perilipin A does not. Differences in protein mobility do not correlate with PAT protein-mediated control of lipolysis catalyzed by HSL or endogenous lipases. Forster resonance energy transfer and co-immunoprecipitation experiments reveal that each of the three PAT proteins bind HSL through interaction of the lipase with amino acids within the highly conserved amino-terminal PAT-1 domain. ADFP and LSDP5 bind HSL under basal conditions, whereas phosphorylation of serine residues within three amino-terminal protein kinase A consensus sequences of perilipin A is required for HSL binding and maximal lipolysis. Finally, protein kinase A-mediated phosphorylation of HSL increases lipolysis in cells expressing ADFP or LSDP5; in contrast, phosphorylation of perilipin A exerts the major control over HSL-mediated lipolysis when perilipin is the main lipid droplet protein. 相似文献
992.
993.
Xiaowen Gong Weiyuan Ye Haibo Zhou Xiaohui Ren Zhigang Li Weiyin Zhou Jun Wu Yicheng Gong Qi Ouyang Xiaolin Zhao Xuejun Zhang 《Acta biochimica et biophysica Sinica》2009,(11):883-891
Acetylcholinesterase (ACHE) expression may be induced during apoptosis in various cell types. Here, we used the C-terminal of AChE to screen the human fetal brain library and found that it interacted with Ran-binding protein in the microtubule-organizing center (RanBPM). This interaction was further confirmed by coimmunoprecipitation analysis. In HEK293T cells, RanBPM and AChE were heterogeneously expressed in the cisplatin-untreated cytoplasmic extracts and in the cisplatin-treated cytoplasmic or nuclear extracts. Our previous studies performed using morphologic methods have shown that AChE translocates from the cytoplasm to the nucleus during apoptosis. Taken together, these results suggest that RanBPM is an AChE-interacting protein that is translocated from the cytoplasm into the nucleus during apoptosis, similar to the translocation observed in case of ACHE. 相似文献
994.
995.
Xiaoyu Guo Guilian Zhang Huili Gong Kaiyun Wang Jintun Zhang 《Frontiers of Biology in China》2009,4(2):222-227
To investigate the dynamic changes in the artificial vegetation in an abandoned mining site, we analyzed the relationships
among community types, environmental variables and community structure in the process of vegetation restoration in the Antaibao
mining site, China by survey of the communities and use of biological dating methods. By means of the quantitative classification
method (two-way indicator-species analysis, TWINSPAN) and the ordination technique (de-trended correspondence analysis, DCA;
and de-trended canonical correspondence analysis, DCCA), the plant communities were classified into seven groups: community
I, Robinia pseudoacacia + Pinus tabulaeformis-Caragana korshinskii-Agropyron cristatum; community II, Robinia pseudoacacia-Hippophae rhamnoides-Artemisia capillaries; community III, Ulmus pumila-Elaeagnus angustifolia-Artemisia capillaries;community IV, Caragana korshinskii-Agropyron cristatum + Artemisia capillaries; community V, Hippophae rhamnoides-Elymus dahuricus; community VI, Elaeagnus angustifolia + Hippophae rhamnoides-Brassica jucea;community VII, Hippophae rhamnoides + Elaeagnus angustifolia-Salsola collina. We conclude that the community types and diversity are mainly influenced by the succession time and the soil organic matter
content. The forest community is more adaptable to the special inhabitation than the shrub community. 相似文献
996.
RuiJie Zhang Xia Li YongShuai Jiang GuiYou Liu ChuanXing Li Fan Zhang Yun Xiao BinSheng Gong 《中国科学:生命科学英文版》2009,52(2):163-172
High-throughout single nucleotide polymorphism detection technology and the existing knowledge provide strong support for
mining the disease-related haplotypes and genes. In this study, first, we apply four kinds of haplotype identification methods
(Confidence Intervals, Four Gamete Tests, Solid Spine of LD and fusing method of haplotype block) into high-throughout SNP
genotype data to identify blocks, then use cluster analysis to verify the effectiveness of the four methods, and select the
alcoholism-related SNP haplotypes through risk analysis. Second, we establish a mapping from haplotypes to alcoholism-related
genes. Third, we inquire NCBI SNP and gene databases to locate the blocks and identify the candidate genes. In the end, we
make gene function annotation by KEGG, Biocarta, and GO database. We find 159 haplotype blocks, which relate to the alcoholism
most possibly on chromosome 1∼22, including 227 haplotypes, of which 102 SNP haplotypes may increase the risk of alcoholism.
We get 121 alcoholism-related genes and verify their reliability by the functional annotation of biology. In a word, we not
only can handle the SNP data easily, but also can locate the disease-related genes precisely by combining our novel strategies
of mining alcoholism-related haplotypes and genes with existing knowledge framework.
Supported by the National Natural Science Foundation of China (Grant Nos. 30570424, 60601010 and 30600367), the National High-Tech
Research and Development Program of China, (Grant No.2007AA02Z329), the Key Science and Technology Program of Heilongjiang
Province(Grant No.GB03C602-4), Natural Science Foundation of Heilongjiang Province (Grant No. F2008-02), Youth Science Foundation
of Harbin Medical University (Grant No. 060045) and Science Foundation of Heilongjiang Province Education Department (Grant
Nos. 11531113 and 1152hq28). 相似文献
997.
Chang Yang Gong Shuai Shi Peng Wei Dong Ling Xiu Zheng Shao Zhi Fu Gang Guo Liang Jing Yang Yu Quan Wei Zhi Yong Qian 《BMC biotechnology》2009,9(1):8
Background
Most conventional methods for delivering chemotherapeutic agents fail to achieve therapeutic concentrations of drugs, despite reaching toxic systemic levels. Novel controlled drug delivery systems are designed to deliver drugs at predetermined rates for predefined periods at the target organ and overcome the shortcomings of conventional drug formulations therefore could diminish the side effects and improve the life quality of the patients. Thus, a suitable controlled drug delivery system is extremely important for chemotherapy. 相似文献998.
Background
Trehalose synthase (TreS) which converts maltose to trehalose is considered to be a potential biocatalyst for trehalose production. This enzymatic process has the advantage of simple reaction and employs an inexpensive substrate. Therefore, new TreS producing bacteria with suitable enzyme properties are expected to be isolated from extreme environment. 相似文献999.
Huiqing Zhan Jan M. Spitsbergen Wei Qing Yi Lian Wu Thomas A. Paul James W. Casey Guor Muor Her Zhiyuan Gong 《Marine biotechnology (New York, N.Y.)》2010,12(6):640-649
A retrovirus homologue gene of cellular cyclin D
1
, walleye dermal sarcoma virus rv-cyclin gene (orf A or rv-cyclin), was expressed in the livers of zebrafish under the control of liver fatty acid-binding protein (lfabp) promoter. To prevent possible fatality caused by overexpression of the oncogene, the GAL4/upstream activation sequence (GAL4/UAS)
system was used to maintain the transgenic lines. Thus, both GAL4-activator [Tg(lfabp:GAL4)] and UAS-effector [Tg(UAS:rvcyclin)] lines were generated, and the rv-cyclin gene was activated in the liver after crossing these two lines. Since no obvious neoplasia phenotypes were observed in the
double-transgenic line, cancer susceptibility of the transgenic fish expressing rv-cyclin was tested by carcinogen treatment. Unexpectedly, transgenic fish expressing rv-cyclin gene (rvcyclin+) were more resistant to the carcinogen than siblings not expressing this gene (rvcyclin–). Lower incidences
of multiple and malignant liver tumors were observed in rvcyclin+ than in rvcyclin– fish, and the liver tumors in the rvcyclin+
group appeared later and were less malignant. These results suggest that expression of rv-cyclin protects the fish liver from carcinogen damage and delays onset of malignancy. These findings indicate that transgenic fish
models are powerful systems for investigating mechanisms of inhibition and regression of liver tumors. 相似文献
1000.
Construction and Preliminary Analysis of a Deep-Sea Sediment Metagenomic Fosmid Library from Qiongdongnan Basin, South China Sea 总被引:1,自引:0,他引:1